RESUMEN
Coronavirus disease 2019 (COVID19), caused by the severe acute respiratory syndrome coronavirus2 (SARSCoV2), led to an outbreak of viral pneumonia in December 2019. The present study aimed to investigate the host inflammatory response signaturecaused by SARSCoV2 in human corneal epithelial cells (HCECs). The expression level of angiotensinconverting enzyme 2 (ACE2) in the human cornea was determined via immunofluorescence. In vitro experiments were performed in HCECs stimulated with the SARSCoV2 spike protein. Moreover, the expression levels of ACE2, IL8, TNFα, IL6, gasdermin D (GSDMD) and IL1ß in HCECs were detected using reverse transcriptionquantitative PCR and/or western blotting. It was identified that ACE2 was expressed in normal human corneal epithelium and HCECs cultured in vitro. Furthermore, the expression levels of IL8, TNFα and IL6 in HCECs were decreased following SARSCoV2 spike protein stimulation, while the expression levels of GSDMD and IL1ß were increased. In conclusion, the present results demonstrated that the SARSCoV2 spike protein suppressed the host inflammatory response and induced pyroptosis in HCECs. Therefore, blocking the ACE2 receptor in HCECs may reduce the infection rate of COVID19.